(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. The separation of common organic osmolytes using a Waters SugarPak column, as described in the Materials and methods. (B) Separation of most major osmolytes common in elasmobranch muscle, as well as several other common organic osmolytes, using a mobile phase of 500 mg l–1 Ca-EDTA. (A) Enlargement of the region of response from the approximate retention times of 12.5 to 21 min, using different concentrations of Ca-EDTA as the mobile phase. Note the retention times of glycine and alanine are reduced as the concentration of Ca-EDTA decreases.