Fig. 2. (A) A trace from the flow cytometer showing the increase in seat patch BCF of a dehydrated (14.1%) B. marinus placed on water at time 0. (B) Mean seat patch BCF values ± 1 s.e.m. from 15 trials (three replicates in five toads) in dehydrated B. marinus; open symbols, BCF in the dry chamber; filled symbols, BCF on water. On water, BCF was significantly lower in B. marinus compared to B. alvarius (P<0.001, Fig. 1B vs Fig. 2B). Values for toads with 50 mmol l^–1 NaCl were not different from DI.