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Figure 5


Fig. 5. Faecal pellets of Temora stylifera male and female copepods. Reconstructed three-dimensional confocal laser scanning microscopy images obtained using both 488 and 543 nm {lambda} lasers to excite fluorescein and chlorophyll, respectively (A,C,E) and observed in transmitted light (B,D,F). (A,B) Faecal pellets produced by copepods fed the dinoflagellate Prorocentrum minimum for 24 h. (A) The red fluorescence is due to chlorophyll. Bar, 61.4 µm. (B) The faecal pellets can be seen to contain mainly Prorocentrum minimum cell walls. (C,D) Faecal pellets produced by copepods fed FitcDx-encapsulating liposomes (LIPOF) for 24 h. The green fluorescence in C is due to FitcDx. Bar, 80.0 µm. (E) Faecal pellets produced by copepods fed a mixed diet of LIPOF and P. minimum for 48 h. In E the green spots are due to FitcDx and the red spots are due to chlorophyll. Bar, 76.5 µm.