Fig. 4. Temora stylifera female copepods. (A,C,E,G) Reconstructed three-dimensional confocal laser scanning microscopy images obtained using both the 488 and 543 nm lasers to excite fluorescein and chlorophyll, respectively. (B,D,F,H) The same copepods as in A,C,E,G, seen in transmitted light. (A,B) A starved female (time=0). In A the chitinous wall is autofluorescent, the yellow colour is the result of superimposition of green and red fluorescence. (C,D) A female fed the dinoflagellate Prorocentrum minimum for 48 h. In C the red fluorescence inside the gut is due to chlorophyll emission. (E,F) A female fed FitcDx-encapsulating liposomes (LIPOF) for 48 h. In E the green fluorescence inside the gut is due to the emission of FitcDx. (G,H) Fluorescent 3-D image of a female fed a mixed diet of LIPOF and P. minimum for 24 h. In G the green fluorescence in the gut does not overlap with the red fluorescence of chlorophyll. Bar, 0.2 mm.