Fig. 3. Alignment and sequence comparison between CionaKChIP and representatives of the four vertebrate KChIP isoforms. The deduced amino acid sequence of CionaKChIP and selected KChIPs belonging to the four mammalian isoforms were aligned using T-Coffee software (Notredame et al., 2000). KChIP subunits and GenPept. accession numbers were: CionaKChIP, AAS00647; KChIP1a, AAL12489; KChIP2a, AAF81755; KChIP3, Q9Y2W7; KChIP4b, NP_079497. Residues that are identical for at least three of the five KChIPs are shown in reverse lettering (white on black). The positions of the four EF-hands are underlined and labelled below the alignment. X,Y,Z,-Y,-X,-Z are the residues that coordinate Ca²⁺ (Bairoch and Cox, 1990). Arrows indicate exon/intron boundaries for CionaKChIP only. Putative phosphorylation sites for protein kinase C (PKC) are indicated by filled circles.