Fig. 8. Western blots showing Na⁺/K⁺-ATPase protein content in (A) foot muscle and (B) hepatopancreas of active and 10-day estivated snails. Representative blots are shown for four independent samples. Band densities were normalized against the combined measured densities of three protein bands stained strongly with Coomassie Blue that did not change between control and estivated states, and histograms show the mean normalized band densities (± s.e.m., N=10 for foot muscle, N=8 for hepatopancreas). The band shown corresponds to 100 kDa, as determined from a standard curve developed using Kaleidoscope protein size markers, and is consistent with the expected alpha subunit size of Na⁺/K⁺-ATPase.