Fig. 6. Thermal denaturation profiles of rWT M. trossulus cMDH and the
mutants V114H and V114N. Samples were held at 42.5°C for the indicated
time before residual activity was measured in triplicate. The rWT M.
trossulus and V114H mutants are not significantly different in stability,
but the V114N mutant denatures at a significantly higher rate than the other
two forms.