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Figure 4


Fig. 4. Arrhenius activation energies (Ea) of M. trossulus, M. galloprovincialis and M. californianus cMDHs, as well as rWT M. trossulus cMDH, mutants V114H and V114N. Vmax values were standardized to 1.00 at 0°C before loge-transformation, to aid in visualizing differences among the slopes. Ea is proportional to the negative slope of the regression relating loge Vmax to the reciprocal of absolute temperature. An analysis of covariance indicates that none of the Ea values are significantly different ({alpha}=0.05).