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Figure 2


Fig. 2. 6 h infusions. Quantitative analysis of V-H+-ATPase in gills from colchicine-treated, NaCl-infused (col-NaCl IF), base-infused (BIF) and colchicine-treated, base-infused (col-BIF) fish. (A) Whole-gill homogenates. (B) Membrane fraction. V-H+-ATPase abundance was significantly greater only in the membrane fraction of BIF (N=4). Representative immunoblots are shown above each panel. The lower-case letters indicate difference levels of statistical significance (1-way ANOVA, Bonferroni's post test). Results are means ± s.e.m.