Fig. 5. (A) Exogenous EPA causes a dose-dependent increase in the generation of BMSF by disrupted B. balanus seminal vesicles/testis incubated at 12°C for 20 min. Products were separated by RP-HPLC at pH 7.0 and quantified as detailed in the Materials and methods. Values are means ± s.d. (B) BMSF production is inhibited by lipoxygenase inhibitors NDGA (20 µmol l^-1) and esculetin (50 µmol l^-1), and by the cytochrome P450 inhibitor, metyrapone (100 µmol l^-1). Representative data are shown from an experiment repeated several times.