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Figure 4


Fig. 4. Effect of acidification on post-HPLC separated products generated from ionophore-challenged barnacle seminal vesicles/testes. (A) The original RP-HPLC separation at pH 7.0 and the fractions collected (green, blue and yellow regions) that were subsequently acidified to pH 3.5, re-extracted and separated again by RP-HPLC at pH 7.0 (B-D).