Fig. 2. Effect of pH prior to solid phase extraction, on the products produced by barnacle seminal vesicles/testis. Chromatograms shown are from RP-HPLC separation at pH 7.0 of material extracted at either pH 3.5 (red trace) or pH 7.0 (black trace). Note the significant reduction in peak 1 (BMSF; A) and the appearance of triene- and pentaene-containing compounds including peaks 2 (B) and 3 (C) following this brief period of acidification. Asterisks indicate peaks with similar UV profiles.