Fig. 6. (A) CRH-R1 and CRH-BP expression levels in gills, (B) the total number of CRH-BP immunopositive cells and (C) CRH-R1 and CRH-BP expression in skin of C. carpio following 24 h restraint. Values are means ± s.d. (N=5). Asterisks indicate significant differences from the controls (P<0.05). Expression is standardised for ribosomal 40S expression and the relative quantification value is expressed as 2^-Ct.