Fig. 7. Effect of surgical treatments on locomotory behaviours. (A) Percentage of the three locomotory behaviours produced spontaneously by leeches in the five experimental groups. Control, N=46; cut, N=9; missing 1, 2, N=10; missing 1, 2, 3, N=10; missing, 1, 2, 3, 4, N=9. (B) Locomotion elicited by moderate touch to the posterior dorsal body. Control, N=46; cut, N=9; missing, 1, 2, N=10; missing, 1, 2, 3, N=11; missing, 1, 2, 3, 4, N=10. Locomotion was much less common in animals with ablations than in controls or animals with nerve cuts (Fig. 6A). Locomotion included only swimming, crawling and incomplete crawling, so the sum of the bars for each experimental condition is 100% and the percentages for the three modes of locomotion are not independent. Within each set of bars (that is, for each behaviour within each panel), conditions that are not significantly different from one another are labelled by the same letter, A, B, C, etc., and bars that are labelled by different letters are significantly different from one another (differences among surgical treatment groups were tested with a Kruskal-Wallis test, and post-hoc comparisons among groups were done using a Tukey's HSD procedure on the ranked data). Bars that are labelled with two or more letters are not significantly different from bars labelled with any of the letters. This analysis includes a total of six extra controls, and eight leeches with ablations were used in only this analysis.