Fig. 2. Cooling chamber used to determine the freezing resistance of G. acuticeps larvae. External ice was applied with a frozen toothpick; internal ice was introduced by touching the caudal peduncle with a cold needle. (A) Cross-section showing the placement of the inlet (IT) and outlet (OT) thermocouples, magnetic stirring bar (MS), false bottom (FB) and the cooling jacket (CJ) through which coolant from a refrigerated bath was circulated. (B) Top view. The larva is approximately 12 mm long.