Fig. 1. Comparison of the cold tolerance of summer-acclimatized larvae and adults and cold-acclimated larvae of the Antarctic midge Belgica antarctica. Three replicates of 10 individuals were transferred from 4°C to the specified temperatures for 24 h. Survival was assessed following a 24 h recovery at 4°C, and all individuals that displayed at least some movement were deemed to have survived. Values are means ± s.e.m.