Fig. 8. Distribution of NKCC in branchial chloride cells of adult D. labrax following direct transfer from seawater to freshwater and exposure to freshwater for 1 day (A,A'), 7 days (B,B') and 14 days (C,C',C''). Note the presence of four different cell types. The SW-type chloride cells (SW-CC; A') are rich in basolateral NKCC. The intermediate cells (arrowheads, C') have moderate amounts of basolateral NKCC, the immunonegative cells (asterisks, B') lack NKCC staining and the FW-type cells (FW-CC, C'') have an apically stained cell pit. F, filament; L, lamellae. Scale bars, 20 µm (A-C), 4 µm (A',B'), 5 µm (C'), 2 µm (C'').