Fig. 6. Distribution of NKCC in adult D. labrax following long-term adaptation in seawater (A,C,E,E') and freshwater (B,D,D',F,F'). (A,B) The anterior intestine (I) is stained along the brush-border membrane and the staining intensity appears stronger in the seawater-acclimated fish. (A). Note the presence of single basolaterally stained cells in both types of fish (A,B, arrows). (C,D) The renal collecting ducts (CD) have a (sub)apical staining (C,D,D') which is stronger in freshwater-acclimated fish (D,D'). Note the clearly distinguishable apical (D' arrow) and subapical (D' arrowhead) immunostaining. (E) Seawater-type chloride cells (S-CC) located on the filaments (F) are basolaterally stained (E'). (F) Freshwater-type chloride cells (F-CC) located on both the filaments and the lamellae (L), are (sub)apically stained (F'). Scale bars, 3 µm (D'), 5 µm (E',F'), 10 µm (A-F).