Fig. 1. The effects of laminarin, PMA and zymosan on PKC phosphorylation. Lymnaea stagnalis haemocytes were challenged with (A) laminarin (10 mg ml^-1), (B) PMA (10 µmol l^-1) or (C) zymosan (10 µg ml^-1) for 0-30 min and their effects on PKC phosphorylation determined by western blotting. Equal amounts of protein were loaded in each lane and membranes were probed with anti-phospho PKC (pan), anti-phospho PKC, or anti-phospho PKCß_II antibodies as indicated in the figure. Anti-actin antibodies were used to confirm equal loading of proteins. Relative PKC phosphorylation levels (shown in the graph in A) detected with anti-phospho PKC (pan) antibodies were determined by image analysis. Values are means ± s.d. from four independent experiments. ^*P0.05 and ^***P0.001 when compared to control values (time 0).