Fig. 5. Expression of sea bream fTnT gene isoforms afTnTsb
(higher molecular mass), LfTnTsb (lower molecular mass) and
18s rRNA assessed by RT-PCR after 7 (64 d.p.h.), 18 (75 d.p.h.) and
31 (89 d.p.h.) days of treatment with T3 and in control animals. Reaction
products fractionated on a 2.5% agarose gel (A) and respective graphical
representation of the relative expression of isoform afTnTsb (B) and
LfTnTsb (C) against 18s; (D) the afTnTsb:LfTnTsb
ratio. The efTnTsb isoform is not represented graphically since no
expression was detected in any treatment at any sampling point.
aSignificant statistical differences between the control and
T3-treated groups.