Fig. 4. Developmental expression of sea bream fTnT isoforms and 18s ribosomal RNA (18s rRNA) assessed by RT-PCR. (A) Reaction products were fractionated on a 2.5% agarose gel. Embryos from 12, 18 and 36 h.p.f., larvae from 1 to 75 d.p.h. and juveniles of 89 d.p.h. were analysed. Adult white (WM) and red (RM) muscle, heart (H) and liver (L) were also analysed. A no template, negative control was used (C-). Three bands are detected which are products of the sea bream fTnTsb gene. (B) Graph showing the average expression of each fTnT isoform in relation to 18s rRNA of three samples. Values are means ± s.e.m. (C) Graph showing the ratio between the different sea bream fTnT isoforms at various stages and in different tissues.