Fig. 3. Developmental expression patterns of (A) GLUT4, (B) GLUT2 and (C) GPDH in Atlantic cod. Atlantic cod were analyzed for GLUT4, GLUT2 and GPDH expression from eggs to larval fish by qRT-PCR. Each value represents a single analysis of a biomass of approximately 100 mg. Expression levels of the target gene were normalized to 18S rRNA. Units represent the relative expression of normalized target gene with respect to a calibrator sample, which in this case is the tissue that expresses the target gene at the lowest detectable level. Sampling times are based on developmental stages and changes in diet. Day 0, 45 degree days; HH, halfway to hatching; Hatch, 105 degree days. Post-hatch samples: Yolk sac, day 2; Rotifers, day 19; Rot/Atr (rotifers/Artemia), day 47; Artemia, day 52; Art/DF (Artemia/dry food), day 54; Dry food, day 60.