Fig. 5. PKC knockout abolishes the effects of H₂O₂ on decrease in lens gap junction dye transfer. Lucifer Yellow and rhodamine-dextran were microinjected as described in the Materials and methods. Confocal microscopy was used to measure the depth of Lucifer Yellow dye transfer (in µm) from the point of injection in the equatorial region of lenses. The movement of the dye through the extracellular spaces was accounted for by subtracting the rhodamine-dextran fluorescence. Each experimental group contained eight 6-week-old lenses; values are means ± s.e.m. ^*Significant difference at P0.05. KO, PKC knockout, PBS, phosphate-buffered saline.