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Figure 1


Fig. 1. Phylogenetic analysis of rainbow trout (Oncorhynchus mykiss) NOS protein. The phylogenetic tree was constructed with commercial software (DNAMAN version 5.2.9; Lynnon Biosoft) using maximum likelihood as the distance method. Support values for nodes (100% in all cases and therefore not shown) were determined through bootstrapping of 100 pseudo-datasets. Branches are drawn to scale as the scale represents replacement of 5% of the amino acids in the protein alignment. Sequences for eNOS were obtained from rabbit (Oryctolagus cuniculus; accession AAO47084), human (Homo sapiens; accession NP_00059), dog (Canis familiaris; NP_001003158), rat (Rattus norvegicus; NP_068610) and mouse (Mus musculus; P70313). Sequences for nNOS were obtained from pufferfish (Takifugu rubripes; accession AAL82736), Japanese medaka (Oryzias latipes; accession BAD11808), zebrafish (Danio rerio; accession AAO53340), rabbit (accession AAB68663), rat (NP_434686), mouse (NP_032738), human (NP_000611) and dog (XP_534695). Sequences for iNOS were obtained from zebrafish (XP_692103), goldfish (Carassius auratus), rainbow trout (CAC8306), rat (CAB46089), mouse (AAH62378) and dog (NP_001003186).