Fig. 2. Northern blot analyses of mRNA isolates from nasal glands of untreated (fw) and osmotically stressed (sw) ducklings using AQP1- and AQP5-specific DIG-labelled cDNA probes. (A) Left panel: example of a northern blot with simultaneous detection of AQP1 and AQP5 mRNAs; right panel: inverse fluorescence image of an ethidium bromide-stained agarose gel used for northern blotting. The positions of residual ribosomal RNAs (28S, 18S) in the mRNA samples loaded onto the gel lanes are indicated. These signals were used to control for even loading of the gels. (B) Relative intensities of northern blot signals in mRNA isolates from nasal glands of untreated (fw) and osmotically stressed (sw) ducklings obtained with AQP1-specific cDNA probes (means ± s.d., N=12, *P<0.05, ANOVA). (C) Relative intensities of northern blot signals in mRNA isolates from nasal glands of untreated (fw) and osmotically stressed (sw) ducklings obtained with AQP5-specific cDNA probes (means ± s.d., N=12, **P<0.01, ANOVA).