Fig. 4. (A) Net Na+ (solid bars) and Cl– (hatched bars)
flux across the isolated intestine increases transiently in killifish, after
transfer to fresh water (FW; black bars) from brackish water (BW, 10%
seawater; grey bars). (B) Unidirectional Na+ influx across the
isolated intestine does not change significantly after fresh water transfer,
but the small non-significant increase is enough to account for the change of
net Na+ flux shown in A. Net absorption occurs in the positive
direction. Values are means ± s.e.m. (N
6). *Significant
difference from pre-transfer (Pre) brackish water control
(P<0.05). Net Cl– flux was found to be greater
than net Na+ flux by two-way ANOVA.