Fig. 2. (A) Example of excitatory junctional potentials (EJPs) recorded from musculature of active anterior foreguts in response to a 10 ms stimulation of the severed recurrent nerve. Following exposure to the active hemolymph factor the amplitude of the EJP dropped significantly but recovered after a saline rinse. (B) We found a significant decrease in the average amplitude of the EJPs recorded from anterior esophageal constrictor muscles of the anterior foreguts (N=10; paired t-test, P<0.001). The average EJP amplitude returned to the initial saline level in the rinse. *Significant difference from both saline and rinse levels.