Fig. 5. Total HSC70/HSP70 protein content prior to (0 min) and at 10, 20, 30 and 60 min following a 36°C heat stress in female and male flies. Results are expressed relative to 5 ng HSP70 protein included as a standard (see Materials and methods). The data were adjusted for block effects of running western blots in groups. Solid red lines and red filled triangles, values for the HN females; solid blue lines and blue filled triangles, LN females; broken red lines and open red triangles, HN males; broken blue lines and open blue triangles, LN males. Values are mean ± s.e.m. of 8 samples (2 samples from each HN_1–4 or LN_1–4 line).