Fig. 3. The effect of a heat-shock pretreatment on T_KD. Each bar represents the difference (in °C) between the mean T_KD of pretreated flies and the mean T_KD of non-pretreated flies for the CN_1–4, LN_1–4, and HN_1–4 lines. The values for the females are shown at the top, males at the bottom. Mean difference in T_KD for females (and for males) from each line was estimated using 500 pretreated flies and 500 non-pretreated flies. Black bars, values for CN_1–4; blue bars, LN_1–4; red bars, HN_1–4.