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Fig. 2. Merged immunofluorescence images of transverse sections of fast muscle at 48 days post-fertilisation. Expression of (A) fast myosin heavy chain (MyHC; green) and fast troponin C (red), (B) fast MyHC (green) and fast tropomyosin (red), (C) fast MyHC (green) and fast myosin light chain 1 (MyLC1; red). Incomplete overlap of the fluorescent markers (arrows) show that fast muscle fibres contain differing proportions of fast-twitch muscle isoform transcripts. Scale bars, 100 µm.