Fig. 1. Experimental setup for assessing flight ability of D. melanogaster. Ten bottles were connected in series to test ten flies simultaneously. Flies were held for 5 min in the Eppendorf tube to acclimate to the treatment. The lid of the tube was then removed with a magnet, releasing the fly into the chamber. We ranked flight performance as one of three categories: flew above the fluon line within 2 min (`flight'), flew within an additional 2 min period while the bottle was gently tapped (`coerced flight'), or failed to fly (`no flight').