Fig. 3. Identification of parvalbumin in kingfish muscle. Upper image is a SyproRuby-stained SDS–PAGE gel showing a pair of bands in the 10–11 kDa range in red and white muscle of kingfish. The lower image is a western blot of the same gel employing an anti-parvalbumin antibody. Both muscle fiber types appear to express the same two parvalbumin isoforms. The larger (upper) isoform has relatively low affinity for the antibody.