Fig. 5. Transcription profiles for crustacean cardioactive peptide (CCAP) mRNA during the moult cycle of Carcinus (A,C,E) and Callinectes (B,D,F). Data are shown as un-normalized (copy no. per thoracic ganglion; A,B), normalized against total RNA (copy no. µg^-1 RNA x10⁶; C,D), and normalized against a housekeeping gene, arginine kinase (AK; copy no. CCAP mRNAx10³/10⁶ copies of AK mRNA; E,F). For Carcinus, N=5-11 independent samples were taken at each moult stage. For Callinectes, N=8-10. Error bars indicate +1 s.e.m.