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Figure 4


Fig. 4. Expression of crustacean cardioactive peptide (CCAP) mRNA in Carcinus maenas tissues. Inset panel shows 430 bp PCR product (using CCAP F1, R1 gene-specific primers) amplified from cDNA derived from reverse transcription of 100 ng total RNA from eyestalk (Es), cerebral ganglion (Cg), circum-oesophageal commissure (Co), thoracic ganglion (Tg), heart (H), hepatopancreas (He), testis (Te) and ovary (Ov) tissues. Numbers to right of panel are sizes of ladder markers (bp). Histogram shows levels of CCAP mRNA from quantitative RT-PCR expressed as copies per µg RNA for Es (N=14), Cg (N=8) and Tg (N=8). Error bars indicate +1 s.e.m. Eyestalk tissues contain significantly more CCAP mRNA than cerebral ganglia (*asterisk, P<0.05, Dunn's multiple comparison).