Fig. 2. Expression of crustacean cardioactive peptide (CCAP) mRNA during embryonic development of Carcinus maenas. Three to 11 independent measurements of different embryo batches were made at each developmental stage. Error bars indicate +1 s.e.m. Developmental stages were defined according to (Chung and Webster, 2004); additionally, the percentage development is indicated in brackets. During early developmental stages (yolk-limb buds), very low, but significant numbers of transcripts were observed, which are shown in brackets.