Fig. 9. The effects of a rapid thermal shift from 26°C to 15°C, which should increase membrane rigidity, on the expression of genes involved in phosphatidylethanolamine (PE) biosynthesis (A; Sply, Cdpet), membrane lipid signaling (B; Pld, Wun) and encoding a putative AcCoAS (C; CG6432). Shown are least square means ± 1 standard error and significant fixed effects of T_Expose(T_Rear) from mixed-model ANOVAs. Units of expression are (1/Ct)x1000. Fold expression changes were calculated from serial dilution standard curves. ^**P<0.01, ^***P<0.001, ^****P<0.0001.