Fig. 7. Population and temperature acclimation effects on activity (A) and expression (B) of the lipid-mediated signaling enzyme, phospholipase D (PLD). Shown are least square means ± 1 standard error and significant fixed effects from mixed-model ANOVAs. Fold expression changes were calculated from serial dilution standard curves. ^*P<0.05, ^****P<0.0001.