Fig. 5. Population and temperature acclimation effects on ethanol tolerance (A), the ethanol catabolic enzymes, ADH (B), AcCoAS (C) and two putative AcCoAS-encoding loci (D). Shown are least square means ± 1 standard error and significant fixed effects from mixed-model ANOVAs. Fold expression changes were calculated from serial dilution standard curves. ^*P<0.05, ^**P<0.01, ^***P<0.001, ^****P<0.0001.