Fig. 2. (A) Northern blot analysis of total RNA extracted from frog testis hybridized with the fRLX cDNA as probe. Each lane contained 20 µg of total RNAs extracted from testis incubated with vehicle alone (Krebs), testosterone (T) and T in combination with CPA. (B) The same filter was hybridized with the fP1 cDNA probe as control. (C) Relative density of the fRLX and fP1 band measured with Gel Doc.