Fig. 2. Expression and localization of AeNHE3 in osmoregulatory organs in larva and adult Aedes aegypti. AeNHE3 is highly expressed in the posterior midgut (PMG) of larval (A) and adult (C) Aedes aegypti. Malpighian tubules (MT) and hindgut (HG) also show high AeNHE3 immunoreactivity (red). In larva, anterior midgut (AMG) expresses lower levels of NHE3 whereas adult AMG expresses levels comparable to PMG. Interestingly, the distal part of adult PMG shows reduced expression of NHE3. In both anterior (D) and posterior (E) midgut, NHE3 expression (red) is restricted to the basolateral membrane. Actin in D and E is stained green. (B) The background fluorescence with preimmune serum used as primary antibody. Immune antibody preabsorbed with the peptide used for immunization gave similar results as in B. Scale bars, 100 µm (B); 50 µm (D,E).