Fig. 6. Stability of Ostf1 transcripts. EGCPC were pre-incubated for 2 h with 5 µg ml^-1 actinomycin D in isosmotic medium (300 mOsmol kg^-1). Treatments were initiated at time 0 for isosmotic controls (black squares) and cells exposed to hyperosmotic stress (600 mOsmol kg^-1, NaCl; open circles). Ostf1 mRNA levels were determined by quantitative real-time PCR and normalized to 18S RNA. Remaining mRNA relative to time 0 is shown on a logarithmic scale. Asterisks indicate significant differences between pairs (P<0.05). Values are means ± s.e.m., N=4.