Fig. 5. Analysis of Ostf1 mRNA transcription rates. Intact fish (A) or EGCPC (B) were exposed to hyperosmotic stress (freshwater to seawater transfer for intact fish, and from 300 to 600 mOsmol kg^-1 exposure for EGCPC). At the indicated times samples were collected and Ostf1 mature (Ostf1) and nascent (hnOstf1) mRNAs were quantified by qPCR. Relative mRNA abundance with respect to control (time 0) and normalized to ß-actin content are depicted. Values are means ± s.e.m., N=3. Asterisks indicate significant differences between Ostf1 and hnOstf1 (P<0.05).