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Figure 4


Fig. 4. Validation and specificity of qPCR primers and reactions. (A) Proposed partial intron/exon structure for tilapia Ostf1. Exons (boxes, E1-3) and introns (lines) are depicted. Location of qPCR primers for the mature (mF and mR) and nascent (hnF and hnR) mRNAs are indicated. (B,C) Gel electrophoresis of the qPCR products (B) and dissociation curve profiles (C). (D,E) Standard curves of qPCR reactions for Ostf1 mRNA (D) and hnRNA (E) obtained with serial dilutions of cDNA. The equation of the linear correlation and the correlation coefficient (R) are indicated.