Fig. 2. Ostf1 mRNA response to different hyperosmotic media in EGCPC. Osmolality
was increased from 300 to 600 mOsmol kg-1 by addition of the
indicated compounds. After 2 h, cells were collected and Ostf1 mRNA quantified
by qPCR. Relative mRNA abundance with respect to control (isosmotic medium)
and normalized to ß-actin content are depicted. Values are means ±
s.e.m. (N=3 experiments). Asterisks indicate significant differences
(P<0.05) relative to the control.