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Figure 7


Fig. 7. Localization of AQP-x5 by immunofluorescent staining in Hyla japonica (A,B), Rana japonica (C,D) and Bufo marinus (E,F). Fluorescence images (A,C,E) for AQP-x5 and the corresponding Nomarski differential interference contrast images (B,D,F) are shown. AQP-x5 (green; arrows); V-ATPase E-subunit (red; arrowheads) are clearly visible. l, lumen. Scale bars, 10 µm (A-D); 50 µm (E,F).