Fig. 6. Immunofluorescence localization of AQP-x5 in the skin glands. (A,C,D) Fluorescence images of AQP-x5 and (B) the corresponding Nomarski differential interference contrast image. (A,B) The labels (green; arrows) are clearly visible in the apical plasma membrane of granular cells in the small granular glands (sg). Weak positive reaction is visible in the apical plasma membrane of glandular cells located in upper sides of the mucous glands (arrowheads). No labeling is seen in the mucous (m) and granular (g) glands. The asterisk in B indicates red blood cells, displaying a nonspecific label for AQP-x5. (C) No labeling is detected in any of the cells of the skins when anti-AQP-x5 is preabsorbed with the corresponding antigen peptide. (D) Double-labeling for AQP-x5 (green) and V-ATPase E-subunit (red); V-ATPase-expressing cells are observed among the glandular cells (arrowheads). Nuclei are counterstained with DAPI (blue); l, lumen. Scale bars, 50 µm (A-C); 10 µm (D).