Fig. 9. Spatial expression of agNAT8 transcript in the alimentary canal, CNS and PNS of An. gambiae larvae. (A) In situ hybridization of agNAT8-specific probes in the whole-mount midgut from 4th instar An. gambiae larvae. (B) Magnified image of the rectal gland. (C) Intensive hybridization in the salivary gland. (D) Stretched-opened esophageal epithelial infolding, cardia; this epithelial region is associated with secretion of the tubular peritrophic membrane. (E) Area-specific labeling in the isolated neuronal ventral cord of CNS; three thoracic ganglia are shown. (F,G) Dorsal and lateral views of agNAT8 hybridization pattern in the larval stemma (eye); this labeling is associated with primary photosensitive neurons (green arrows). (H) An example of hybridization of sensory neurons associated with vibration-sensitive hair sensilla on the larval head (black arrow). (I) Labeling in the basal part of the peg sensillum at the head capsule (white arrow); this structure has an unknown sensory role in larvae, but it is morphologically similar to temperature-sensitive structures in adults. (J) agNAT8-pecific hybridization of specific populations of chemosensory neurons in maxillary pulp (MP) and labium afferents (LB) (open arrows). AMG, anterior midgut; CA, cardia, a sub-esophageal invagination; GC, gastric caeca; MT, Malpighian tubules; PMG, posterior midgut. All images were acquired using a Hoffman contrast microscope except for A and E, which were acquired using a stereo microscope. Bars, 1000 µm (A), 200 µm (B-E), 100 µm (F-H,J), 50 µm (I).