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Figure 7


Fig. 7. Kinetic properties of agNAT8 transport mechanism. (A) The effects of increasing phenylalanine concentration on agNAT8-mediated currents; values are means ± s.e.m.; N ≥3. (B) The effects of increasing sodium ion concentration on phenylalanine-elicited, agNAT8-mediated currents; values are means ± s.e.m.; N ≥3, except for L-Met, N=1. (C) E0.5 for substrates with highest apparent transport velocity; values are means ± s.d.; N ≥3. (D) Estimated Hill coefficient for selected substrates; values are means ± s.d.; N ≥3. Lines in A and B represent iterative nonlinear regression of the data with three-parameter Hill function (E[S]0.5 and {eta}[S] are incorporated in the graph panels; see inserts).