Fig. 2. Alignment and reconstruction of agNAT8 structure. (A) Sequence/structure alignment of characterized insect transporters relative to the first crystallized bacterial NAT from A. aeolicus, LeuT_Aa (Yamashita et al., 2005). (B) 2D structure of agNAT8 based on structural homology with the LeuT_Aa protein sequence. NCBI Accession no.: LeuT_Aa, NP_214423 (PDB no., 2A65); msKAAT1, AAC24190; msCAATCH1, AAF18560; aeAAT1, AAR08269; agNAT8, AAN40409. Filled and open stars represent putative cationic gates at extra- and intracellular interfaces, respectively. Squares indicate putative substrate binding sites; red and blue spheres outline sites, which interact with the first and second sodium ion respectively. Red and yellow boxes show putative glycosylation motifs and disulfide bridges, respectively. The 12 transmembrane domains are numbered 1-12.