JEB -- Ahmed et al. 209 (16): 3101 Figure IG4

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Fig. 4. Changes in pHi upon challenging cells with hypertonicity while using (a) Ca²⁺-containing medium (control), (b) Ca²⁺-free medium and (c) Ca²⁺-free medium along with preincubation of cells with the intracellular Ca²⁺-chelating agent BAPTA-AM. Values are means ± s.e.m. of 68-95 cells from 5-10 independent preparations. Inset: pHi changes upon exposing cells to 25 µmol l^-1 BAPTA-AM followed by Ca²⁺-free medium. Values are means ± s.e.m. of 46 cells from three independent preparations.